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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-99902-306

Pfu DNA Ligase, Agilent Technologies

Catalog Number: (99902-306)
Supplier: Agilent Technologies
Description: Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage ...
Product Image-99900-316

Gigapack III Packaging Extracts, Agilent Technologies

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency a...

Product Image-102969-096

NEB PCR Cloning Kit (Without Competent Cells), New England Biolabs

Catalog Number: (102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism ...

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Product Image-77506-810

BioXp® Select DNA Cloning Gibson Assembly Kits

Supplier: TELESIS BIO
Description: The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.

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Product Image-102877-564

NEB® PCR Cloning Kit, New England Biolabs

Catalog Number: (102877-564)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechan...

SDS Small Business Enterprise


Product Image-76081-600

Ampligase® Thermostable DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: Highly specific thermostable DNA ligase for high-temperature applications

Vendor Image-EM70189-3

pT7Blue Perfectly Blunt® Cloning Kit, Novagen®, EMD Millipore

Supplier: MilliporeSigma
Description: The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of ...

Certificates

Product Image-97065-204

NxGen™ T4 DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This e...

Vendor Image-EM70550-3

T7Select®10-3 Cloning Kit, MilliporeSigma

Catalog Number: (EM70550-3)
Supplier: MilliporeSigma
Description: Novagen's T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7.

Certificates


Vendor Image-EM71229-3

pSTBlue-1 Perfectly Blunt® Giga Cloning Kit, EMD Millipore

Catalog Number: (EM71229-3)
Supplier: MilliporeSigma
Description: The pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency (>1×109 cfu...

Certificates


Product Image-PAM1801

T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-102500-052

Gibson Assembly Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Gibson Assembly was developed by Dr

Small Business Enterprise

Product Image-101227-656

QUICK LIGATION™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

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Product Image-102969-104

NEBNext Ultra II Ligation Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-ta...

Small Business Enterprise

Product Image-101228-152

Taq DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotid...

Small Business Enterprise

Product Image-102500-174

T7 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Small Business Enterprise

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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